complete methylcellulose medium methocult tm h4434  (STEMCELL Technologies Inc)

Journal: International Journal of Molecular Sciences

Article Title: Gallic Acid Enhances the Efficacy of BCR::ABL1 Tyrosine Kinase Inhibitors in Chronic Myeloid Leukemia through Inhibition of Mitochondrial Respiration and Modulation of Oncogenic Signaling Pathways

doi: 10.3390/ijms25147958

Figure Lengend Snippet: GI decreases colony formation and induces apoptosis in CML stem/progenitor cells. ( A , B ) GI inhibits the colony formation of primary CML CD34 + cells in a dose-dependent manner and to a greater extent than normal cord blood CD34 + cells. Colonies were counted under a microscope and the results are presented as relative to the control. Four CML patient samples and three healthy cord blood samples were included in this study. ( C ) GI also induces apoptosis dose-dependently in primary CML cells after 72 h. All data were conducted in independent triplicates. * indicates p < 0.05 and ** indicates p < 0.01.

Article Snippet: Primary CD34 + cells at 1–5 × 10 3 were plated in StemMACS TM HSC-CFU complete methylcellulose medium w/o Epo (Miltenyi Biotec, Bergisch, Germany).

Techniques: Microscopy, Control

Journal: International Journal of Molecular Sciences

Article Title: Gallic Acid Enhances the Efficacy of BCR::ABL1 Tyrosine Kinase Inhibitors in Chronic Myeloid Leukemia through Inhibition of Mitochondrial Respiration and Modulation of Oncogenic Signaling Pathways

doi: 10.3390/ijms25147958

Figure Lengend Snippet: GI and GA exhibit synergistic inhibitory effects with BCR::ABL1 TKIs in both CML cell lines and stem/progenitor cells. ( A – D ) Combination index (CI) was calculated using the method of Chou and Talalay and CI < 1 indicates synergism, CI = 1 is additive and CI > 1 suggests antagonism. GI and GA demonstrated synergy with imatinib in both LAMA84 and K562 cell lines with CI < 1 at both 50% and 90% growth inhibition. Data are representative of the mean and SD of at least three independent experiments. ( E ) GI significantly enhanced with the inhibitory effect of dasatinib on the colony formation of CML CD34 + (n = 3). In total, 20nM of dasatinib was used. All data were conducted in independent triplicates. * indicates p < 0.05.

Article Snippet: Primary CD34 + cells at 1–5 × 10 3 were plated in StemMACS TM HSC-CFU complete methylcellulose medium w/o Epo (Miltenyi Biotec, Bergisch, Germany).

Techniques: Inhibition